Improvement of Method for Evaluating  Amylose Content in Rice at Low Generations of Breeding

doi:10.3969/j.issn.1001-7216.2010.01.16

Abstract

Abstract: A simple iodine staining method for evaluating amylose content in rice was developed. Amylose content in rice was divided into four levels from low to high, according to the colors from brown red to dark blue after staining. The evaluation on the amylose content in 28 rice varieties by using the method showed that there was a significant correlation between the staining results and standard value (r = 0.95**). The method was applied to the improvement of rice amylose content in low generations, and the accuracy of the method in Wx gene screening was verified by molecular marker assistant selection. The amylose content evaluated by this method was highly coincident with rice starch synthesisrelated gene markers in 22 breeding parents for restorer and maintainer lines of hybrid rice.

Key words: rice, amylose content, quality improvement, low generation, staining method, molecular marker assistant selection, breeding

摘要： 针对稻米直链淀粉含量在育种低世代遗传改良中的问题，提出了简易的碘蓝染色法：采用一定量的半粒糙米染色，按照染色后颜色由棕红到深蓝的差异，将稻米直链淀粉含量分为4个类别，分别对应的直链淀粉含量由低到高。使用该方法测定了28份供试水稻品种，染色结果与实测值呈极显著相关（r = 0.95**）。利用低世代遗传群体的研究，并通过分子标记辅助验证了该方法对Wx基因筛选的准确性。22份恢复系与保持系育种亲本通过此法所得直链淀粉含量与稻米淀粉合成相关基因标记之间的符合率较高。

关键词: 水稻, 直链淀粉含量, 品质改良, 低世代, 染色法, 分子标记辅助选择, 育种

WANG Yue-xing,NI Shen,CHEN Hong-qi, LIU Gui-fu,YANG Jun, DUAN Bin-wu,ZHU Xu-dong. Improvement of Method for Evaluating Amylose Content in Rice at Low Generations of Breeding[J]. Chinese Journal of Rice Science, DOI: 10.3969/j.issn.1001-7216.2010.01.16 .

王跃星,倪深,陈红旗,刘贵富,杨军,段彬伍,朱旭东,. 稻米直链淀粉含量的低世代筛选方法研究 [J]. 中国水稻科学, DOI: 10.3969/j.issn.1001-7216.2010.01.16 .

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Improvement of Method for Evaluating Amylose Content in Rice at Low Generations of Breeding

稻米直链淀粉含量的低世代筛选方法研究

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